Cephahbus parvus as a carrier of Rhizobium japonicum in field experiment on soybean cultures
نویسندگان
چکیده
Two methods of soybean seeds inoculation by Rhizobium japonicum have been compared in a field experiment : i) traditional use of inoculated turf embedding the seeds; ii) use of Cephalobus parvus as bacterial carrier (3 O00 and 300 O00 nematodes per linear meter). There is no difference in yield between the inoculation methods in spite of a greater number of bacterial germs provided by nematode inoculation probably because there is a limit to the number of bacteria that can be used by one soybean seed. Any bacterium above this limit cannot be used by the seed. With turf inoculation al1 the nodules are localized at the root collar whereas with nematode inoculation they are regularly distributed on the rootlets. This is beneficial in case of soil surface drying which destroys nodules at the root collar.
منابع مشابه
Determination of Hydrogenase in Free-living Cultures of Rhizobium japonicum and Energy Efficiency of Soybean Nodules.
A sensitive tritium exchange assay was applied to the Rhizobium system for measuring the expression of uptake hydrogenase in free-living cultures of Rhizobium japonicum. Hydrogenase was detected about 45 hours after inoculation of cultures maintained under microaerophilic conditions (about 0.1% O(2)). The tritium exchange assay was used to screen a variety of different strains of R. japonicum (...
متن کاملRole of Lectins in Plant-Microorganism Interactions: III. Influence of Rhizosphere/Rhizoplane Culture Conditions on the Soybean Lectin-binding Properties of Rhizobia.
The influence of rhizosphere/rhizoplane culture conditions on the ability of various rhizobia to bind soybean seed lectin (SBL) was examined. Eleven strains of the soybean symbiont, Rhizobium japonicum, and six strains of various heterologous Rhizobium species were cultured in root exudate of soybean (Glycine max [L.] Merr.) and in association with roots of soybean seedlings which were growing ...
متن کاملVariation in Symbiotic Performance of Bradyrhizobium Japonicum Strains and Soybean Cultivars under Field Conditions
In this study the symbiotic interactive effect of different Bradyrhizobium japonicum strains with six soybean cultivars were evaluated under fi eld conditions. The rhizobial strains ASR011, USDA123 and CB1809 respectively showed hostcultivar specifi city with JS335, Lee and Bragg. B. japonicum ASR011 recorded the highest nodulation and nitrogenase activity with all the studied cultivars. Genera...
متن کاملEffects of Rhizobium– Mycorrhizae Symbiosis and Foliar Application of Urea on Some Traits of Soybean
In order to investigate the effect of rhizobium bacteria, mycorrhiza fungi and foliar application of urea on qualitative and quantitative traits of soybean an experiment was conducted based on randomized complete block design with four replications. The experiment included six treatments as: Control (C), Rhizobium bacteria (R), Rhizobium bacteria + Mycorrhiza fungi (R+M), Rhizobium bacteria + F...
متن کاملModified Fluorescent Technique, Using Rhodamine, for Studies of Rhizobium japonicum-Soybean Symbiosis.
Rhodamine-conjugated capsular polysaccharides isolated from Rhizobium japonicum 61A76NS were used to examine binding between the labeled polysaccharides and soybean roots. Fluorescein-labeled polysaccharides were not satisfactory because soybean root hairs autofluoresce in the fluorescein region.
متن کامل